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Mary Ward Microbeam Laboratory
The UCC Mary Ward Microbeam Laboratory is a new high-spec analytical facility located in the Cooperage building (Room G12A) that offers cross-platform options for surface analyses, including imaging surface structure at the micro- to nano-scale, bulk analysis of sample chemistry, plus chemical imaging.
The lab also provides a service to all academic departments within UCC and also to the broader community of scientists working in the earth-, environmental, materials, chemical and bio-sciences and to industry. For access to the instrumentation in the Mary Ward Laboratory, and to learn more about applications, please contact the laboratory Manager Prof. Maria McNamara (maria.mcnamara@ucc.ie) or the laboratory Experimental Officer Peter Chung (peter.chung@ucc.ie).
Click on the images of the equipment below to find out more.
JEOL JSM-100 VP-SEM
The JEOL IT-100 variable pressure (VP)-SEM is equipped with a backscatter detector, dedicated VP-secondary electron detector and a 30 mm2 EDS detector. This equipment allows quick, routine imaging and analysis of sample structure and chemistry at a sub-mm to nanometer scale.
The secondary electron detector (SED) is used for imaging of sample structure at the micrometre- to nanometre scale, with an ultimate resolution of < 20 nm at 20 kV on gold-coated samples.
The backscatter electron (BSE) detector allows chemical imaging of specimens with a mean atomic mass variation of ca. 0.1.
The equipment can run in VP mode (> 30 Pa) in conjunction with the environmental SE and BSE detectors, which allows (fully non-destructive) analysis of non-coated samples. TEM sections can be checked and analysed using a dedicated STEM holder.
The EDS detector provides standardless quantification and advanced X-ray mapping of elements heavier than (and including) carbon. Chemical mapping allows sample chemistry to be correlated with sample microstructure.
Sputter-coating of samples can be performed at the bench using our in-house sputter coater fitted with an Au/Pd target.
The Perkin Elmer Spotlight 400i near- and mid-IR FTIR microscope is coupled to a Frontier FTIR instrument and is equipped with a high-resolution imaging accessory and extra-large sample stage. The equipment offers quick and routine analysis of bulk sample chemistry and chemical imaging of molecular composition at a micron-scale; especially useful for polar materials (e.g. with O-, N-, S-bonds).
Spatial resolution for chemical maps is 6 µm in standard micro-ATR/transmission/reflectance mode.
The dedicated high-resolution imaging accessory provides enhanced spatial resolution of 1.56 µm pixel size for maps up to 1 mm x 1 mm.
The extra-large sample stage allows movement in X, Y and Z planes and can accommodate irregularly-shaped samples up to A4 in size and up to ca. 25 mm thick
This Raman microscope is a flexible research-grade instrument that includes unique real-time focus tracking capabilities. It is equipped with two diode-pumped solid-state lasers (DPSS) with wavelengths of 532 nm and 785 nm coupled to a high-performance Raman spectrometer.
The laser spot size is continuously variable from 1 to 300 µm (objective and excitation wavelength dependent) with fully optimised beam path achieving high levels of confocality (up to 2.5 µm depth resolution).
High spectral performance is assisted by an automated, kinematically mounted, magnetically attached, Rayleigh line rejection filter set for 532 nm excitation, using paired filters, allowing ripple-free measurement of the Raman spectrum to 100 cm-1 from the laser line. It can resolve spectral features narrower than 0.5 cm-1, and can detect minute shifts in Raman band position (as low as 0.02 cm-1).
For accuracy and sensitivity, the CCD array is a near infrared enhanced, deep depletion detector (1024 x 256 pixels), Peltier cooled to minus 70°C (-70°C).
LiveTrackâ„¢ focus-tracking technology allows the production of chemical images (maps) from samples with uneven surfaces with the function to view in 3D.
Additional software allows ultra-fast mapping with speeds up to 1000 spectra per second with StreamHRâ„¢&²Ô²ú²õ±è;Rapide and the Particle Analysis module can derive detailed statistical information from chemical images.
The Leica Ultracut UC7 Ultramicrotome is used to prepare consistently high-quality freestanding semi- and ultrathin sections of soft materials for electron microscopy or to generate perfectly smooth, polished block face surfaces for light microscopy, AFM or chemical characterization via e.g. FTIR or Raman spectroscopy.
The fully motorized knife stage minimizes user-related vibration and allows delivery of consistent sample thickness of 1 nm – 15 µm.
The control unit allows quick adjustment of feed, cutting window, cutting speed and illumination parameters during sectioning. The vibration-decoupled gravity-stroke cutting arm allows chatter-free sectioning.
Semi-thin sections can be stained at the bench, dried on a hotplate and checked for sample structure.
The Leica DM 2700 epi-illumination microscope is coupled to an Ocean Optics 2000+ spectrophotometer aligned with the microscope C-axis to provide easy measurement of spectral reflectance of macroscopic and microscopic materials.
A beam splitter allows the microscope to be operated with all light directed to the eyepieces or split between the eyepieces and C-mount.
The microscope is fitted with 5x, 10x, 20x, and 100x objective lenses.
An inbuilt diaphragm allows reduction of the size of the area from which reflected light is collected and analysed to approx. 15 µm wide.
The spectrophotometer can be easily swapped with a Leica digital camera for photography of analysed regions.
Spectra can be calibrated against white and mirror standards for hue and reflectance, respectively. Left- and right-circularly polarized filters allow materials to checked for circular polarization.
A versatile, modular, digital microscope with a large range of magnifications to 2500x with 1600 x 1200 pixel resolution, and the ability to generate scalable 3D images and real-time stitched images.
This equipment allows quick and easy sputter coating of samples with a thin film (< 5 nm thick) of Au or Au/Pd to reduce sample charging during high-vacuum SEM analysis.
This microscope has a wide base and extra long working distance to accommodate thick samples. A ring light plus fully adjustable fibre optic lights provide a high level of control over lighting, including the production of extremely low angle light for surface features with low relief.
The Perkin Elmer TurboMatrix HS-40 Headspace Trap Sampler allows the solvent-free extraction of volatile compounds using precision thermostatting and pressure-balanced technology. The headspace trap increases analytical sensitivity by 100 times compared to a standard standalone GC-MS. This equipment allows monitoring of high-temperature stability reactions for specific biomolecules in solid or liquid samples up to 15 ml. The Clarus 590 GC-MS features a flame ionisation detector (FID) for separation and identification of molecular fragments.
